Calf intestinal alkaline phosphatase neb
WebProduct Name: Alkaline Phosphatase Calf Intestinal (CIP) Cas.# None SECTION 2–COMPOSITION/INFORMATION ON INGREDIENT 1. Glycerol 50% Cas.# 56-81-6 … WebMar 9, 2015 · Calf intestinal alkaline phosphatase (CIP) CIP buffer: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM dithiothreitol, pH to 7.9 at 25ºC Protease inhibitor cocktail, EDTA-free...
Calf intestinal alkaline phosphatase neb
Did you know?
WebAlkaline phosphatase from calf intestine hydrolyzes 5′-monophosphate groups from both DNA and RNA. It can also hydrolyze 5′-diphosphate and 5′-triphosphate groups from RNA. Application Use this preparation of calf intestinal alkaline phosphatase to remove 5′-terminal phosphates from DNA or RNA and immunoprecipitated RNA samples. [ 2] [ 3] [ … WebQuick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Rapid and irreversible heat inactivation eliminates unwanted...
WebApr 10, 2024 · During the clinical and scientific research of cell genetics, it had found that the ribosomal DNA (rDNA) region could be a safe and effective gene targeting site for foreign genes .Given on the basis ,for the purpose of vertifying this theory in vivo ,We construct a new generation vector smid targeting Mouse ribosomal DNA (rDNA),pMrn … WebJun 4, 2024 · The phosphatase can be added directly into the digestion reaction during or after DNA digestion Add 1 μl of Quick CIP for every 1 pmol of DNA ends (about 1 μg of a 3 kb plasmid) and incubate at 37°C for 10 minutes Quick CIP is active in all NEB restriction enzyme buffers
Webtion enzymes, T4 DNA ligase, calf intestinal alkaline phosphatase, Pfu and Taq DNA polymerases were purchased from NEB (USA). Gene cloning kits, PCR primers, mutagenic oligonucleotides (oligo hereaf-ter), chlortetracycline (cTc) and other chemicals were ordered from Sangon (China). cTc was dissolved in WebThe phosphatase can be added directly into the digestion reaction during or after DNA digestion CIP is active in all NEB restriction enzyme buffers DNA purification is required …
WebShrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source. Rapid and irreversible heat inactivation eliminates unwanted activity Improved storage stability …
WebLigation was carried out at 15 °C for 18 hours using T4-ligase (NEB, EuroClone, Pero, Italy). Linear DNA constructs were further purified on agarose gel and then inserted into the pPICZa vector, opportunely double-digested, and dephosphorylated with calf intestinal alkaline phosphatase (CIP; NEB, EuroClone, Pero, Italy). god s country rock versionWeb2 rows · A Pichia pastoris clone that carries the calf intestinal alkaline phosphatase gene from ... A Pichia pastoris clone that carries the calf intestinal alkaline phosphatase gene … god scripts robloxgodscroft foxholeWebCalf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme … gods creative power for healing charles cappsWebDatasheet for Alkaline Phosphatase Calf Intestinal (CIP) (M0290; Lot 0641404) - DocsLib religation of linearized plasmid DNA. The enzyme Reagents Supplied with Enzyme: Functional Assay: Dephosphorylation with CIP of Alkaline acts on 5´ protruding, religation of linearized plasmid DNA. booking thunWebCalf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA. Concentration: 20 units/µL. Applications: Dephosphorylation of 5´-phosphorylated … gods creation of the universeWebCalf intestinal alkaline phosphatase (CIAP) catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´-termini. Revised 4/18. Complete Protocol PDF … god screen background