2024 Grf gif wheat

Grf gif wheat

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August undefined, 2024

WebNov 17, 2024 · Identification of TaGRFs. A total of 30 TaGRF genes (including 13 homoeologous groups) were finally identified from the wheat genome using nine Arabidopsis GRF and 12 rice GRF genes as input sequences. The lengths of TaGRF proteins ranged from 211 to 611 aa and the mean value was 359 aa; the molecular … WebJan 11, 2024 · Rice Growth-Regulating Factors (GRFs) were originally identified to be gibberellin (GA)-induced, but the nature of GA induction has remained unknown because most reports thereafter focused on revealing their roles in growth-promoting activities. GRFs have the WRC (Trp, Arg, Cys) domain to target DNA and contain the QLQ (Gln, Leu, …

Genome-Wide Identification and Analysis of the Growth

WebMar 1, 2024 · This GRF-GIF technology performed well under various transformation conditions and protocols and an antibiotic resistance marker was not required due to the … WebAug 24, 2024 · Among the three wheat GIF cofactors, ... Finally, we show that a dicot GRF–GIF chimera improves regeneration efficiency in citrus, suggesting that this strategy can be applied to dicot crops. magic kingdom tips and tricks

JIPB Plant Biology - Wiley Online Library

WebMar 3, 2024 · With slight modifications reported here, the standard protocol can be applied to the hexaploid wheat “Cadenza” and the tetraploid durum wheat “Kronos” with efficiencies of up to 4% and 10%, respectively. The system has also been employed to assess the developmental gene fusion GRF - GIF with outstanding results. WebMicroRNA miR396, GRF transcription factors and GIF co-regulators: a conserved plant growth regulatory module with potential for breeding and biotechnology Multicellular life relies on complex regulatory mechanisms ensuring proper growth and development. WebOct 16, 2024 · Interestingly, when GRF and GIF were introduced as two independent genes on a single construct, the positive benefit was greatly reduced as compared to the expression of the single chimeric gene. The … magic kingdom tour guide

Plantae A GRF–GIF chimeric protein improves the …

GRF-GIF Chimeras Boost Plant Regeneration: Trends in Plant Science

WebFeb 1, 2024 · The miR396–GRF/GIF module. The GRF family of transcription factors was found to exist in a wide variety of land plants from liverworts to angiosperms, and recently also in charophyte algae, the closest living relatives to all land plants [reviewed in Ref. 9 •].GRFs are defined by the combination of two specific conserved domains – the QLQ … WebJan 25, 2024 · The evolutionary history was inferred by using the maximum likelihood method (Supplemental Fig. 1). A BLAST search with wheat and grape GRF and GIF genes was used to identify GRF and GIF genes in pepper (Capsicum annuum); pepper GRF protein sequences were aligned with GRF protein sequences from citrus, grape, … magic kingdom tickets one dayWebAn efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.). Plant Methods 15:121. doi: 10.1186/s13007-019-0503-z. … magic kingdom ttc center

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Grf gif wheat

GRF–GIF chimeric proteins enhance in vitro regeneration and

WebSep 2, 2024 · The combination of distinct morphogenetic factors, the maize BABY BOOM transcriptional regulator (ZmBBM/EREB53) and the wheat GRF4-GIF1 (GROWTH REGULATING FACTOR4 - GRF-INTERACTING FACTOR1) chimera, together with a modified QuickCorn protocol, regenerated transformed maize seedlings in approximately … WebThe growth-regulating factors (GRFs) are plant-specific transcription factors. They form complexes with GRF-interacting factors (GIFs), a small family of transcriptional co-activators. In Arabidopsis thaliana, seven out of the nine GRFs are controlled by microRNA miR396. Analysis of Arabidopsis plan …

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WebDec 1, 2024 · GRF-GIF regulates the transition between stem ... as observed in GRF4-GIF1 expressing "chimeras" from wheat (Debernardi et al., 2024). The GFR-GIF chimera … WebAug 16, 2024 · The overexpression of a chimeric protein consisting of the GRF4 and GRF-interacting factor 1 (GIF1) proteins reinforce the regeneration efficiency and regeneration speed in wheat, triticale,...

WebOct 12, 2024 · To generate the different wheat GRF-GIF chimeras, we obtained the coding sequences of GRF1, GRF5, GRF9, GIF2 and GIF3 by gene synthesis. Then, we generated the different chimeras (GRF1-GIF1, GRF5-GIF1, GRF9-GIF1, GRF4-GIF2, and GRF4-GIF3) by overlapping PCR following the same strategy described to generate GRF4-GIF1. All … WebOct 10, 2024 · For example, mTaGRF4-TaGIF1 can generate a chimera that confers a two fold to nine fold boost in regeneration in wheat and is linked to the ability of GRF-GIF to …

WebAll wheat GIF (TaGIF) proteins have highly conserved SNH and QG (Gln, Gly) domains. The wheat GRF (TaGRF) gene family has 13 pairs of segmental duplication genes and no tandem duplication genes; the TaGIF gene family has two pairs of segmental duplication genes and no tandem duplication genes. WebTo generate the different wheat GRF-GIF chimeras, we obtained the coding sequences of GRF1, GRF5, GRF9, GIF2 and GIF3 by gene synthesis. Then, we generated the …

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WebJul 8, 2024 · The closest homologs to wheat GRF4 and GIF1 are highlighted in orange for citrus and in violet for grape. We will refer to those genes and their encoded proteins as citrus GRF4 (Ciclev10032065m), citrus GIF1 (Ciclev10022144m), and grape GRF4 (GSVIVT01024326001) and grape GIF1 (GSVIVT01036262001). magic kingdom tron coasterWebOct 12, 2024 · We generated a citrus chimera and a heterologous grape GRF–GIF chimera using the closest homologs to wheat GRF4 and GIF1 … magic kingdom touring plan with genie plusWebtransformation efﬁciency in wheat (Debernardi et al., 2024). Therefore, we employed the same strategy using our system, designing a new vector called pGH ‐rGRF‐GIF (Figure 1A,B). We transformed “TC” watermelon with the pGH‐GRF‐GIF, pGH‐ rGRF‐GIF, and pGH vectors to compare ef ﬁciencies (Figure 1A). magic kingdom under constructionWebMar 3, 2024 · With slight modifications reported here, the standard protocol can be applied to the hexaploid wheat “Cadenza” and the tetraploid durum wheat “Kronos” with … magic kingdom t shirtWebDec 11, 2024 · Different software may use files with the same extension for different types of data. 8 known uses of the GRF file extension. Byline Graphic Configuration. … magic kingdom toursWebOur menu offers something for everyone: creamy shakes, onion rings, crispy chicken tenders, awesome salads or nachos. Whether it’s a team celebration, family get together, … magic kingdom tours priceWeb3 52 The GRF proteins form complexes with GIF cofactors that also interact with chromatin remodeling 53 complexes in vivo 15, 16.Multiple levels of regulation control the efficiency of the assembly of 54 functional GRF/GIF complexes in vivo 17.Loss-of-function mutations in GIF genes mimic the 55 reduced organ size observed in GRF loss-of-function mutants or … magic kingdom vacations packages