Hcd2 reporter mice
WebFoxp3hCD2 mice via the lytic anti-CD25 antibodies (clone PC61, Figure S4 A) as previously described [ 18, 22 ]. Treatment with PC61 significantly reduced %CD4 + hCD2 + Treg in the spleen and circulation (Figure S4 B-C). At 4 weeks after CI to a P3 heart, Treg depletion contributed to a significantly increase in cardiac fibrosis (Figure S5 A-B). WebMurine Treg cells were isolated from spleen and lymph nodes of wild-type C57/BL6 mice or Foxp3-hCD2 reporter mice, sorted to high purity via flow cytometry and transduced during expansion with α-CD3/CD28 stimulation and interleukin-2 (IL-2). Treg cells could be stably expanded under these conditions ( Fig. 1 B ).
Hcd2 reporter mice
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WebJun 18, 2024 · The fluorescent ubiquitination-based cell-cycle indicator (Fucci-492) mice were from the Riken BioResource Center (Ibaraki, … WebApr 16, 2013 · To generate the Aid-cre-cd2 construct, an expression cassette of cre IRES hCD2, the intracellular domain of which was deleted, was inserted into exon 2 of mouse …
WebApr 25, 2015 · Behavioural analyses. Using the open field novel object recognition (NOR) test and spontaneous alternations in the Y-maze, Aldh2-/-mice showed a progressive … WebLMP2A flSTOP mice were then crossed to CD19-Cre; LMP1 flSTOP mice, which express LMP1 and a human CD2 (hCD2) reporter from the Rosa26 locus and a Cre recombinase that activates transgenes in late pro-B cells . CD19-Cre; LMP1/LMP2A flSTOP mice died on day 4 or day 5 after birth with 100% penetrance.
WebApr 13, 2024 · A new report holds clues. April 13, 2024 3:26 PM. By: Max Barnhart. Microscope images of urinary tract cells from mice that were not given a UTI (naïve) … WebTransgenic mice expressing the codon-improved Cre (iCre) 37 under the control of either the vav promoter elements or hCD2 LCR were generated. To test the efficiency of Cre …
WebApr 1, 2024 · A novel knock-in model p16hCD2 mouse expressed the surface marker hCD2, which correlated with the induction of p16 INK4a. ... We herein generated a new reporter mouse model, in which the gene encoding human CD2 (hCD2) was knocked into the endogenous p16, Cdkn2a locus. This mouse possesses the hCD2 transgene …
WebSep 9, 2011 · The hCD2 − group ( Fig. 4 C, squares) initially included four mice that received hCD2 − MoFlow-sorted DBYT cells combined with further ablative anti-hCD2 − to ensure that removal of Foxp3 + cells was optimized. going to qcatWebIn B6.Foxp3 hCD2 mice, the hCD2_CD52 fusion protein appears to be a highly accurate reporter of Foxp3 + CD4 + cells (>98% of Foxp3 + cells are hCD2 +; Fig. S1 B), allowing … going to qldWebThe most common of these reporter mice is the R26R-LacZ, where loxP-transcriptional termination-loxP precedes the β-gal gene at the ubiquitously expressed ROSA26 locus. 7,8 Because unanticipated Cre activity can vary between mice, it is imperative that such analyses extend to testing multiple different mice expressing the Cre transgene ... going to put it in the want ads songs youtubeWebApr 17, 2015 · Mice transgenic for iCre under control of the human CD2 promoter ( hCD2-iCre) are a popular tool to conditionally delete genes in immature thymocytes, mature T cells and B cells without reported effects … going to pure gym for the first timeWebJul 16, 2024 · Phenotypic analysis of the individual Id2 BFP, Gata3 hCD2, and Rora Teal strains revealed minimal perturbation to hematopoietic cell populations (Figures 1 A and S2–S4).Consistent with previous reports of Id2 reporter strains (Jackson et al., 2011, Klose et al., 2014), Id2-BFP expression was observed in all mature ILC subsets, LTi cells, and … going to prom with friendsWebFeb 17, 2024 · FACS analysis for BrdU + B cells was performed on day 7 after tamoxifen treatment ( n = 3 for N2IC mice, n = 2 for CAR mice). g Percentages of BrdU/Ki67 double positive cells within reporter... going to puerto rico in octoberWebApr 17, 2015 · Our results confirm the known activity patterns for both Cre transgenes and unveil additional hCD2-iCre mediated reporter gene recombination in common lymphoid … hazelhurst regional gallery \\u0026 arts centre