Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue-cyan fluorescent light around an emission spectrum maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510–540 nm range. Hoechst stains can be excited with a xenon- or mercury-arc lamp or with an ultraviolet laser. There is a considerable Stokes shift between the excitation an… NettetAbstract Dyes that bind to DNA, such as Hoechst 33342, are commonly used to visualize chromatin in live cells by fluorescence microscopy. A caveat is that the probes themselves should not perturb cellular responses and under normal conditions the dyes are generally non-toxic.
INSTRUCTIONS Hoechst 33342 - Thermo Fisher Scientific
NettetMax Planck Institute of Molecular Cell Biology and Genetics Bethany Unger Hoechst33342 is cell permeable and usually used in live cell imaging. However for long term imaging, … Nettet100 nM 5-HMSiR-Hoechst. Living cells stained for 1h at 37°C in DMEM + 10% FBS and no washing of the probe applied before imaging. Each frame data set consisted of 10000 frames acquired at 100 Hz with excitation light intensity 18 kW/cm2. Reconstruction performed with SVI Huygens Localizer. Video S3. highlander sweatshirt
Frontiers Modelling the erythroblastic island niche of ...
Nettet3 timer siden · Cells respond to perturbations such as inflammation by sensing changes in metabolite levels. Especially prominent is arginine, which has known connections to the inflammatory response. Aminoacyl ... Nettet25. okt. 2024 · Live cell imaging. THP-1 human monocytic cells [] were cultured at 37 °C in 10% fetal calf serum (FCS; Life Technologies, Carlsbad, CA, USA) supplemented Roswell Park Memorial Institute (RPMI) medium 1640 (Thermo Fisher Scientific, Waltham, MA, USA) containing 100 U ml −1 penicillin and streptomycin, respectively.For live cell … NettetA human is, according to the most recent estimates, an assortment of 3.7±0.8×10 13 cells (BNID 109716), plus a similar complement of allied microbes. The identities of the … how is discord used