Pls1rom
Webb26 juni 2024 · Restriction sites of the multicloning sequence (MCS) of pLS1ROM and pLS1OM vectors are indicated. Unique restriction sites in pLS1ROM-GFP, pLS1ROM … Webb2 dec. 2024 · The platform was finally introduced into a new beaker containing 60 mL of medium inoculated with L. monocytogenes Scott A [pLS1ROM-GFP] and incubated for …
Pls1rom
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Webbsequence of pLS1ROM plasmid (accession number: JN381945.1) [22]. Gene synthesis method was applied by synthesizing reverse complementary short DNA oligos using an automatic DNA synthesizer firstly, then short double-strand DNA fragments were obtained by anneal and joined by an one-step thermocycled in vitro recom-bination system []. WebbWe report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae. The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter P M of the S. pneumoniae malMP operon, …
Webb22 mars 2024 · In the presence of sub-inhibitory novobiocin concentrations, which inhibit gyrase activity, higher doubling times were observed in a strain lacking staR, and in two strains in which StaR was over-expressed either under the control of the ZnSO 4-inducible P Zn promoter (strain ΔstaRP Zn staR) or of the maltose-inducible P Mal promoter (strain … WebbLOCUS JN381945 6805 bp DNA circular SYN 25-JUL-2016 DEFINITION Cloning vector pLS1ROM plasmid pLS1ROM, complete sequence. ACCESSION JN381945 VERSION …
Webb1 juni 1984 · The pneumococcal mal recombinant plasmid pLS70, which carries two strong promoters for transcription, could not be transferred and maintained intact i… Webb149 was obtained in a previous work (Puga et al. 2024) using plasmid pLS1ROM–GFP 150 (Ruiz-Masó et al. 2012) for transformation. In this case, the transformed strain was 151 cultured on TSB plates supplemented with 5 µg/mL erythromycin from a stock culture 152 kept at –80 ºC in TSB supplemented with 15% (v/v) glycerol. 153 2.2.
Webb22 mars 2024 · Europe PMC is an archive of life sciences journal literature. This website requires cookies, and the limited processing of your personal data in order to function.
Webb1 jan. 2012 · The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the … inappropriate roblox shirtWebb5 dec. 2014 · S. pneumoniae R6 was grown in AGCH medium (Lacks, 1968) with 0.3 sucrose and 0.2% yeast extract at 37°C in static water bath, and supplemented with … inchconnachan islandWebbfmicb-07-02164 January 7, 2024 Time: 14:56 # 1 ORIGINAL RESEARCH published: 10 January 2024 doi: 10.3389/fmicb.2016.02164 Edited by: Yuji Morita, Aichi Gakuin University, Japan inchconnachan island scotlandWebb2.3.1. pLS1ROM-MCS derivatives . Seven putative pneumococcal TA genes from strain Hungary19A-6 (GI: 169832377) (Chan et al., 2012) were verified in the S. pneumoniae … inchdarnie st boswellsWebbPor favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/61079 inchcruin islandWebbrecombinant plasmid pLS1ROM-GFP upon maltose induction. In contrast to the previously constructed pLS1RGFP plasmid, pLS1ROM-GFP exhibits structural and segregational … inchconnachan wallabiesWebb1 jan. 2012 · The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter P M of the S. pneumoniae malMP operon, followed by a multi-cloning site sequence aimed to facilitate the insertion of target genes. inchdairnie house kinglassie