2024 Raw-blue cells

Raw-blue cells

Author: jgwf

August undefined, 2024

WebIn the present study, we used murine macrophage RAW-Blue cells, which are chromosomally integrated with secreted embryonic alkaline phosphatase (SEAP) inducible by NF-κB. We confirmed the expression of TLR4 mRNA and protein in RAW-Blue cells by RT-PCR and Western blot, respectively. WebRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the activation of the endogenous MIP-2 promoter. MIP‑2, also known as CXCL2, is the murine homolog of human IL-8, a chemokine produced in an NF-κB-dependent manner [3].

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WebFeb 17, 2024 · Coculture D1 Cells:RAW-Blue Cells Additionally, we prepared cocultures of D1 and RAW-blue cells using transwell inserts without the Dex P-A hydrogel. After 24 h incubation at 37 °C, NF-κB/AP-1 activation upon the previously mentioned stimuli was assessed by measuring the release of secreted embryonic alkaline phosphates (SEAPs) … WebJun 5, 2013 · RAW-Blue™ cells are permanently transfected with an NF-κB- and AP-1-sensitive promoter-driven alkaline phosphatase. NF-κB and AP-1 binds to the promoter upon nuclear translocation, and induces the expression of alkaline phosphatase that is detected by a dye-based assay and a plate reader. lime green hair dryer with green light

OxLDL or TLR2-induced cytokine response is enhanced by oxLDL ... - PubMed

WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ... WebApr 22, 2015 · S2 Fig. RAW-BLUE cells detect trace levels of endotoxin in heparin lots. RAW-BLUE cells were cultured with 10 different lots of heparin (#1–10) in the presence or absence of 100pg/mL endotoxin for 24h. TNF-α levels were measured by ELISA in cell supernatant at 24 h. Each point represents mean ± SD of triplicate cell culture. WebRAW-Blue™ cells endongenously express various PRRs, including TLRs (except TLR5), RIG-I, MDA-5, NOD1/2 and Dectin-1 at different levels. The PRR stimulation using specific agonists leads to the activation of NF-kB and AP-1 and, subsequently to the expression of the reporter gene encoding SEAP. RAW-Blue™ cells are selectable with Zeocin®. hotels near jolly grant airport dehradun

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WebNational Center for Biotechnology Information WebRAW-Blue cells were treated with the shown concentrations of FP20 and incubated for 16–18 h. MPLA and LPS (100 ng/mL) were used as controls. The 100% stimulation has been assigned to the positive control LPS. Data are expressed as mean ± SEM of at least three independent experiments ... lime green graphic shirtsWebOct 1, 2024 · Results to characterize the IFN response time of the RAW-Blue ISG ™ (RAW-Blue) and B16-BlueIFN-α/β™ (B16-Blue) cells to IFNα and IFNβ and SEAP catalyzed Quantiblue™ (QB) development time are shown in Fig. 1, Fig. 2, Fig. 3.In Fig. 1 a range of IFN concentrations of 1 to 10,000 Unit/ml (U/ml) were added to each cell type and incubated … hotels near jomo kenyatta intl airport

"WebJan 21, 2024 · Raw-blue™ cells were cultured at 37 °C using manufacturer recommendations (InvivoGen) in a 24-well microplate until 70–80% confluence (1 × 10 5 cells/well) was reached. This cell line expressed the secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by the transcription … " - Raw-blue cells

Raw-blue cells

WebRAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. InvivoGen has developed RAW-Blue ™ cells and RAW-Lucia ™ cells, which express a secreted inducible ... WebJun 28, 2024 · QUANTI-Blue colorimetric enzyme assay: RAW-Blue cells were added at 1 × 10 5 cells per well in a 96-well plate and sequentially the same amounts of different particles at a particle: cell ratio ...

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WebSep 5, 2024 · RAW-Blue cells and HEK 293 h-TLR transfected cells Murine RAW-Blue, HEK-BLUE-hTLR2 and HEK-BLUE-hTLR4 cells carrying a SEAP reporter construct were purchased from InvivoGen (San Diego, CA, USA). WebThe in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor ...

WebRAW-blue cells were pretreated with 200μM Tamiflu, 100μg/mL MMP9i, or 100μg/mL BIM-23127 for 30min followed by 10μg/mL imiquimod or left cultured in media. Cells (1×107 cells) are pelleted ... WebTherefore, RAW-Blue™ cells can be used as: TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels NOD reporter cells: …

WebRAW-Blue™ cells were derived from the murine RAW 264.7 macrophages with chromosomal integration of a SEAP reporter construct inducible by NF-κB. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including all toll-like receptors (TLRs) except for TLR5, but with TLR4 and TLR8 expressed at the highest levels. WebJun 29, 2024 · RAW-Blue macrophages were purchased from InvivoGen (San Diego, CA). All cells were propagated in RPMI 1640 supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 °C in a 5% CO 2 incubator [16, 17]. Cell viability assay. Cell death was quantified by the MTT reduction assay. Cell viability was quantified by the Alamar …

WebSep 30, 2024 · Supernatant from the treated cells was collected and SEAP production was detected over a period of 45 minutes at 655 nm using the QUANTI-Blue ™ detection medium. Detection of protein expression by immunoblotting and densitometry. Cells from the treated well-plate were lysed and saved in the -80 ° C freezer for

WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. lime green graphic t shirtsWebCo-immunoprecipitation experiments using cell lysates from RAW-blue cells further dem- onstrated that Neu1 forms a complex with TLR9 receptors in naive and ODN treatment of cells for 30 min (Fig. 4C). hotels near jolly roger amusement parkWebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced … hotels near jollyville txWebRAW-Blue™ ISG cells allow the detection of bioactive murine type I IFNs or PAMPs by monitoring the activation of the interferon regulatory factor (IRF) pathway. They are derived from the RAW 264.7 macrophages. RAW-Blue™ ISG cells stably express a secreted embryonic alkaline phosphatase (SEAP) gene under the control of the interferon-stimulated lime green graphic teeWebRAW 264.7. TIB-71 ™. RAW 264.7 is a macrophage cell line that was established from a tumor in a male mouse induced with the Abelson murine leukemia virus. Product category. lime green halo headlightsWebSep 6, 2024 · RAW-Blue and THP-1-Blue cells were plated at 1x10 6 cells/ml in flat bottom 96-well plates in a 100 µL volume. Excipients and TLR ligands were added for a final volume of 200 μL. After 24 h of stimulation, supernatants were collected and NF-κB activation was measured using the QUANTI-Blue detection medium according to manufacturer … lime green grommet cloth shower curtainsWebSize. 3-7 x 10e6 cells. Contact us. Ask RAW ™ cells selection audit. Properties. Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water. Conjugation. Blue Substrate. lime green hand blown glass vases